Antimicrobial resistance (AMR) continues to be a global threat to human and animal health. Bacteria carrying resistance genes are present in, and spread between, people, animals, food, and the environment and so a One Health approach to tackling AMR is needed, with all sectors working together to affect change. This third publication of the One Health Report brings together antibiotic resistance and consumption data from humans and animals, combined with data on antibiotic resistance in food and antimicrobial substances in the environment. The data presented here is from 2014 to 2019, the last calendar year before the COVID-19 pandemic, which resulted in delays to publication.Much of this data has already been published separately. The value of this report is that by bringing together existing data, it allows us to better interpret the usage and resistance patterns across all sectors: humans, animals, food, and the environment. This in turn helps us better understand the drivers of AMR in different sectors and how they may interact, allowing us to prioritise the public health risks and areas for intervention. In this report, there are some new additions highlighting emerging areas of work in companion animals and the environment, which are needed to develop our understanding of AMR transmission between people and animals.